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Analysis of genetic diversity and population structure in Argentine and Bolivian Creole cattle using five loci related to milk production Genet. Mol. Biol.
Lirón,J.P.; Ripoli,M.V.; De Luca,J.C.; Peral-García,P.; Giovambattista,G..
Data from five protein-coding loci related to dairy production were used to study the genetic diversity and population structure of Argentine and Bolivian Creole cattle breeds. Genomic DNA was extracted from blood samples of six Creole cattle breeds: Argentine (n = 230), Patagonian (n = 25); "Saavedreño" (n = 140), "Chaqueño Boliviano" (n = 30), "Yacumeño" (n = 27), and "Chusco" (n = 11). kappa-casein, beta-lactoglobulin, growth hormone and prolactin were measured by PCR-RFLP, while alphaS1-casein was typed by PCR-ASO. The results are discussed, focusing on: historical origin, recent differentiation and selection events, Zebu gene introgression, and population structure. This work shows that: (i) For the studied genes, the observed gene frequency profiles...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Creole cattle; Polymorphism; Genetic diversity; Molecular markers.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400010
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Methylation-specific PCR analysis in Col8A1 promoter in Creole cattle carrier of rob(1;29) Electron. J. Biotechnol.
Postiglioni,Alicia; García,Cristina B; Rincón,Gonzalo; Arruga,M. Victoria.
Robertsonian translocation (rob(1;29)) is the most frequent structural chromosomal abnormality in cattle. Heterozygous carriers have a normal phenotype but show a 3-5% decrease in fertility. Chromatin decondensation was evaluated similar to the inactive X chromosome when submitted to demethylating agent. Based on this result, and the concept that imprinted genes are essential in embryonic development, we decided to query genes located on BTA1 and BTA29 that could undergo genome imprinting. The collagen typeVIII-α1 (Col8A1) acted on extracellular matrix structural proteins. DNA bisulfite conversion and sequentiation methods were used to compare its differential methylation patterns. It was performed on eight Creole cattle DNA blood samples from normal and...
Tipo: Journal article Palavras-chave: Bisulfite; Collagen; Creole cattle; Cytosines; Methylation.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000300013
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Polymorphism of locus DRB3.2 in populations of Creole Cattle from Northern Mexico Genet. Mol. Biol.
Fernández,Ilda G.; Ramírez,José Gonzalo Ríos; Vázquez,Amanda Gayosso; Arvizu,Raúl Ulloa; Morales,Rogelio A. Alonso.
The polymorphism of locus BoLA-DRB3.2 of the Major Histocompatibility Complex was evaluated in two northern Mexican Creole cattle populations, Chihuahua (n = 47) and Tamaulipas (n = 51). The BoLA-DRB3.2 locus was typed by amplification and digestion with restriction endonuclease enzymes (PCR-RFLP). Fifty-two alleles were detected (28 previously reported and 24 new ones). In the Chihuahua population, 18 alleles and 5.5 effective alleles were found, while in the Tamaulipas population there were 34 and 10.8, respectively. The allele frequencies ranged from 0.011 to 0.383 in Chihuahua and from 0.010 to 0.206 in Tamaulipas. The frequencies of the new alleles in both cattle populations were low (0.010 to 0.053). The expected heterozygosity was 0.827 and 0.916,...
Palavras-chave: BoLA-DRB3.2; Polymorphism; Creole cattle; PCR-RFLP.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500013
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